Welcome to the

Kollman Lab

Welcome to the

Kollman Lab

Structural studies of intracellular organization

We are examining the structures of the complex macromolecular machinery of cellular organization, with a focus on bacterial cytoskeletal systems and organelles.  Our structural approach centers on cryo-electron microscopy, which we combine with X-ray crystallography.  This integrative approach allows us to generate mechanistic insights over a broad range of size and resolution scales.

Most recent publications:

CTP synthetase assembles filamentous polymers, which we and our collaborators show are enzymatically inactive.  This represents a novel mechanism of enzyme regulation.  Our cryo-EM structre, at 8.4 Å resolution provides insight into the mechanisms of filament assembly and enzyme inhibition.

 

Barry RM, Bitbol AF, Lorestani A, Charles EJ, Habrian CH, Hansen JM, Li HJ, Baldwin EP, Wingreen NS, Kollman JM, Gitai Z (2014) Large-scale filament formation inhibits the activity of CTP synthase. eLife 2014;10.7554 [PMID 25030911][pdf]

In the free form the adaptor protein AlfB disrupts AlfA filament assembly and bundling, but the AlfB-DNA complex promotes filament assembly and tracks the end of growing filaments

 

Polka JK, Kollman JM, Mullins RD (2014)  Accessory factors promote AlfA-dependent plasmid segregation by regulating filament nucleation, disassembly, and bundling [PMID24481252] [pdf]

A review of bacterial actins, focusing on their stuctural and functional diveristy.

Ozyamak, E, Kollman JM, Komeili A (2013)  Bacterial actins ans their diverisity.  Biochemistry 52, 6928-6939.  [PMID24015924]  [pdf]

 

3640 University St., Room W309  Montreal, Quebec, Canada H3A 0C7  phone (514) 398-1716